Journal News

Detecting biomarkers for deadly lung disease

Meric Ozturk
Nov. 21, 2023

Researchers don’t yet know the precise cause of idiopathic pulmonary fibrosis, or IPF, but they know it’s deadly. Patients usually survive about three years after diagnosis. A sudden and unpredictable increase in IPF symptoms, such as shortness of breath and dry cough, could be fatal. Thus, early diagnosis and monitoring are crucial.

Lysophosphatidic acid, or LPA, is produced during lipid metabolism. In the past, researchers have reported that the LPA levels increase in lung fluid following lung injury in mice genetically altered to have pulmonary fibrosis. Deleting one receptor, LPA1, protected mice from fibrosis, indicating that LPA receptors have a role in IPF and may be a potential therapeutic and diagnostic target.

A group led by scientists from Genentech Inc. is now studying whether bioactive lipid species can predict IPF progression. “We had initially been working on chronic obstructive pulmonary disease, COPD,” Genentech senior scientist Margaret Neighbors said. “We compared gene expression levels between COPD patients and healthy patients.”

In this experiment, the gene autotaxin, or ATX, was overexpressed in COPD, and the team already knew that ATX has a role in the LPA signaling pathway, Neighbors explained. ATX generates most of the LPA detected in blood and inflamed tissues, and previous studies showed the role of the ATX–LPA signaling pathway in lung fibrosis in animal models. The team used targeted mass spectrometry to quantify LPA subspecies and assess how it affected COPD exacerbation.

“Then, we turned our focus to another lung disease, IPF, in which autotaxin has a role,” Neighbors said. “We used the same mass spectrometry approach to determine the role of bioactive lipids in IPF for this study.”

They profiled lipid metabolism beyond LPA to compare more lipid species associated with the disease, then combined this with protein biomarker data to learn more about the IPF phenotype than in previous studies.

Neighbors and her colleagues worked with data from about 100 IPF patients between 40 and 80 years old. “Having a rich and diverse cohort helped us to examine how the different lipid species associated with individual clinical measures of IPF,” she said. “This individualistic approach can shed more light to better understand detailed connections between molecular pathways and each clinical manifestation.”

The researchers found that IPF patients had significantly higher levels of five LPA subspecies, and disease in patients with higher levels of one of these, LPA 20:4, worsened sooner than in patients with lower LPA 20:4. Also, patients with higher levels of LPAs have greater declines in diffusion capacity of carbon monoxide, which indicates decreased oxygen capacity of the lungs, an association that has never before been published.

“Our findings suggest LPA subspecies can play roles in pulmonary fibrosis through multiple pathways, rather than any key signal pathobiology,” Neighbors said. “Our study adds a road to the existing lipid map.”

In this study published in the Journal of Lipid Research, the researchers established the association of LPAs with IPF progression. Using mass spectrometry, they characterized lipid dysregulation in IPF. They hope to replicate these findings with additional cohorts and then to observe the molecular mechanisms behind increasing LPA subspecies expressions and their association with IPF.

“We are trying to connect LPAs to clinical manifestations that might be downstream of pathobiologies,” Neighbors said. “Association between higher levels of LPA and declined levels of DLCO (diffusing lung capacity for carbon monoxide) is one new road on this lipid map.”

Enjoy reading ASBMB Today?

Become a member to receive the print edition four times a year and the digital edition monthly.

Learn more
Meric Ozturk

Meric Ozturk is a Ph.D. student in biochemistry at Iowa State University and an ASBMB Today volunteer contributor.

Get the latest from ASBMB Today

Enter your email address, and we’ll send you a weekly email with recent articles, interviews and more.

Latest in Science

Science highlights or most popular articles

Melissa Moore to speak at ASBMB 2025
ASBMB Annual Meeting

Melissa Moore to speak at ASBMB 2025

April 2, 2025

Richard Silverman and Melissa Moore are the featured speakers at the ASBMB annual meeting to be held April 12-15 in Chicago.

 A new kind of stem cell is revolutionizing regenerative medicine
Feature

A new kind of stem cell is revolutionizing regenerative medicine

April 1, 2025

Induced pluripotent stem cells are paving the way for personalized treatments to diabetes, vision loss and more. However, scientists still face hurdles such as strict regulations, scalability, cell longevity and immune rejection.

Engineering the future with synthetic biology
ASBMB Annual Meeting

Engineering the future with synthetic biology

March 31, 2025

Learn about the ASBMB 2025 symposium on synthetic biology, featuring applications to better human and environmental health.

Scientists find bacterial ‘Achilles’ heel’ to combat antibiotic resistance
Webinar

Scientists find bacterial ‘Achilles’ heel’ to combat antibiotic resistance

March 28, 2025

Alejandro Vila, an ASBMB Breakthroughs speaker, discussed his work on metallo-β-lactamase enzymes and their dependence on zinc.

Host vs. pathogen and the molecular arms race
ASBMB Annual Meeting

Host vs. pathogen and the molecular arms race

March 28, 2025

Learn about the ASBMB 2025 symposium on host–pathogen interactions, to be held Sunday, April 13 at 1:50 p.m.

Richard Silverman to speak at ASBMB 2025
ASBMB Annual Meeting

Richard Silverman to speak at ASBMB 2025

March 27, 2025

Richard Silverman and Melissa Moore are the featured speakers at the ASBMB annual meeting to be held April 12-15 in Chicago.