ASBMB Annual Meeting

Challenged by a pandemic, Harbig pursues a new approach to treating flu

Meet a JBC Herbert Tabor Early Career Investigator Award winner

When Anne Harbig found out she had won an award for her very first authored paper, she was shocked.

Harbig-445x522.jpg
Anne Harbig

"It totally caught me by surprise," Harbig said. The paper was the culmination of all of her data from her Ph.D. research in Eva Böttcher-Friebertshäuser's lab at the Marburg Institute of Virology. "I always wanted to deepen my knowledge about viruses, and I was able to have this great Ph.D. project. I'm very grateful for my supervisor."

Harbig's research takes on the challenge of finding proteases that activate specific strains of influenza, which could be targeted with drugs in order to prevent infection and treat the disease. If inhibiting proteases proves effective in halting influenza infection, these methods could be used to target other enveloped viruses as well, including coronaviruses.

Harbig's project came with all kinds of challenges —wading through piles of transcriptome data, hundreds of in vitro studies with proteases that never activated, and now, the global COVID-19 pandemic that introduced all kinds of disruptions as she continues to pursue the project as a postdoc.

"Sometimes, especially on a Friday when you have the results of the week's experiments and things are not working and everything sucks," she said, "it's good to have the weekend to take a break and have ideas for how to do it differently next week."

To stop flu infection, target host enzymes

Anne Harbig seeks to develop targeted treatments that prevent influenza viruses from entering potential host cells. For a flu infection to begin, the virus must have its surface protein called hemagglutinin cleaved by host enzymes known as proteases.

"A protease is a protein, an enzyme, that cleaves other proteins at specific sites," Harbig explained.

Because the virus cannot enter a cell without a protease present, Harbig and her colleagues are identifying proteases involved in activating certain flu strains so they can develop drugs that can block them.

Harbig used RNA sequencing to search for any segments of mRNA coding for proteases found in the lower respiratory system of mice. Once she identified an array of lung proteases, each one was tested individually for its ability to split apart the hemagglutinin on the surface of influenza B viruses. The proteases that were able to do so were narrowed down further to four candidates that activated influenza B infection in mouse cells.

"The idea is, if we know the protease that activates our virus, we can use protease inhibitors to prevent infection," Harbig said. "If we know all the proteases involved, we can test the inhibitors in murine models, in vivo, and if they work there, then maybe later [we can test them] in human studies."

Harbig has earned her Ph.D. and is continuing to work on the project, hoping to see it reach human clinical trials.

Enjoy reading ASBMB Today?

Become a member to receive the print edition four times a year and the digital edition weekly.

Learn more
Guananí Gómez–Van Cortright

Guananí Gómez–Van Cortright is a teacher and freelance science writer.

Get the latest from ASBMB Today

Enter your email address, and we’ll send you a weekly email with recent articles, interviews and more.

Latest in People

People highlights or most popular articles

Being a whole person outside of work
Hobbies

Being a whole person outside of work

Nov. 1, 2024

Creating art, community service, physical exercise, theater and music — four scientists talk about the activities that bring them joy.

‘We’re thankful for our reviewers’
Journal News

‘We’re thankful for our reviewers’

Oct. 31, 2024

Meet some of the scientists who review manuscripts for the Journal of Biological Chemistry, Journal of Lipid Research and Molecular & Cellular Proteomics.

In memoriam: Bruce Ames
In Memoriam

In memoriam: Bruce Ames

Oct. 28, 2024

He invented a cheap and easy way to assess mutagenicity that helped identify many environmental and industrial carcinogens; it became known as the Ames test.

Honors for DebBurman, Margaryan and Santiago–Frangos
Member News

Honors for DebBurman, Margaryan and Santiago–Frangos

Oct. 28, 2024

The Council on Undergraduate Research honors Shubhik DebBurman with a mentoring award. Anush Margaryan wins a Projects for Peace grant to teach refugees in Armenia. UPenn names Andrew Santiago–Frangos an endowed assistant professor.

In memoriam: William L. Smith
In Memoriam

In memoriam: William L. Smith

Oct. 21, 2024

He served as associate editor of both the Journal of Biological Chemistry and the Journal of Lipid Research and was an ASBMB member for more than 40 years.

Honors for Fleming, Garcia and Lichtenstein
Member News

Honors for Fleming, Garcia and Lichtenstein

Oct. 21, 2024

Karen Fleming elected president of Biophysical Society. The Eastern Analytical Symposium honors Ben Garcia for achievements in mass spec. Tufts University names Alice Lichtenstein a distinguished professor.