New Directions in Enzymology

Theme organizers:


Kate S. Carroll
The Scripps Res. Inst.

  Liz Hedstrom
Brandeis Univ.


New Reactions of Old Cofactors

Sunday, March 29, 2015, 9:45 AM - 12:00 PM • Room 258A

Follow the conversation: #enzyme

 Chaired: C. Fierke

9:45                        Chair’s Introduction.

9:50      93.1          Cofactor Dynamics and Ammonia Selectivity in GMP Reductase L. Hedstrom. Brandeis Univ.

10:15                      Fresh Surprises from the Old Cofactor NAD(P)H R.G. Rosenthal, B. Vögeli, G. Stoffel and T.J. Erb. ETH Zurich. (573.9)

10:30    93.2          The Mechanistic Enzymology of Thiamin Biosynthesis T.P. Begley. Texas A&M Univ.

10:55                      An Old Cofactor in a New Light: Adenosylcobalamin in Light-Dependent Gene Regulation M. Jost, S. Padmanabhan, M. Elíaz-Arnanz and C.L. Drennan. MIT, Rocasolano Phys. Chem Inst., Spanish Natl. Res. Council and Univ. of Murcia, Spain. (573.25)

11:10                      Radically New Methylation Reactions in Antibiotic Biosynthesis: Insights into the Mechanism of B12-Dependent Radical SAM Enzymes O. Berteau, S. Pierre, A. Benjdia, C. Gherasim and R. Banerjee. INRA, Jouy en Josas and Univ. of Michigan Med. Sch. (573.39)

11:25    93.3          NaturalProduct Biosynthesis – A Treasure Trove of Multitalented Flavoenzymes B. Moore. Scripps Inst. of Oceanography and Skaggs Sch. of Pharm. and Pharmaceut. Sci., UCSD.

11:50                      Discussion.

Discovering New Enzymes

Monday, March 30, 2015, 3:55 PM - 6:10 PM • Room 258A 

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Chaired: H. Lin

3:55                        Chair’s Introduction.

4:00      237.1       Specificity and Promiscuity in Enzyme Superfamilies K. Allen and D. Dunaway-Mariano. Boston Univ. and Univ. of New Mexico.

4:25                        Functional Characterization of Structural Genomics Proteins in the Crotonase Superfamily C.L. Mills, P. Yin, P.J. Beuning and M.J. Ondrechen. Northeastern Univ. (573.18)

4:40      237.2       Pseudoenzymes as Activators of Enzyme Function: The Prozyme Paradigm in Trypanosomatid Polyamine Metabolism M.A. Phillips. Univ. of Texas Southwestern Med Ctr.

5:05                        Extending the Kynurenine Pathway to an Aldehyde Disarming Enzyme: Mechanistic Study of Bacterial AMSDH and Identification of the Correct Human Enzyme I. Davis, L. Huo, F. Liu, B. Andi, S. Esaki, H. Iwaki, Y. Hasegawa, A.M. Orville and A. Liu. Georgia State Univ., Brookhaven Natl. Lab. and Kansai Univ., Japan. (573.3)

5:20                        Protein Function Prediction Using ProMOL and PyMOL K.N. Hart, T.M. McKay, W. Tedla-Boyd, J.L. Mills, H.J. Bernstein and P.A. Craig. Rochester Inst. of Technol. and Dowling Col., NY. (722.5)

5:35      237.3       The Discovery of New Enzymes in the Human Gut Microbiome F.M. Raushel and M.E. Hobbs. Texas A&M Univ.

6:00                        Discussion.

Enzyme Modules and Complexes

Tuesday, March 31, 2015, 9:45 AM - 12:00 PM • Room 258A 

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Chaired: K. Allen

9:45                        Chair’s Introduction.

9:50      360.1       Quaternary Structure and Activity Modulation in Human Ribonucleotide Reductase F.J. Asturias, E.J. Brignole, K-L. Tsai, C.L. Drennan and J. Stubbe. The Scripps Res. Inst. and MIT.

10:15                      Substrate Specifity and Quaternary Structure of a Novel Class of Tryptophan Synthases F. Busch and R. Sterner. Univ. of Regensburg. (573.5)

10:30    360.2       Quaternary Structure Dynamics in Allostery – Application to Mammalian Phenylalanine Hydroxylase E.K. Jaffe. Fox Chase Cancer Ctr., Temple Univ. Hlth. Syst.

10:55                      Dramatic Conformational Flexibility of Carbon Monoxide Dehydrogenase/Acetyl-CoA Synthase Revealed by Electron Microscopy E.J. Brignole, E.C. Wittenborn, S.M. Thompson, S.W. Ragsdale and C.L. Drennan. MIT and Univ. of Michigan. (573.37)

11:10                      Dynamic Regulation of p97 ATPase Activity by p37 and p47 Is Impaired in Pathogenic p97 IBMPFD/ALS Mutants X. Zhang, L. Gui, T-F. Chou, D. Wong and D. Moen. Harbor-UCLA and LABioMed, Torrance. (891.2)

11:25    360.3       Mass Spectrometry in Structural Biology: Surface-Induced Dissociation/Ion Mobility of Protein Complexes V.H. Wysocki, R. Quintyn, S. Harvey, Y. Song, J. Yan, Y. Ju, A. Tanimoto and A. Sahasrabuddhe. The Ohio State Univ.

11:50                      Discussion.

Strategies for Substrate Recognition

Wednesday, April 1, 2015, 1:15 PM - 3:30 PM • Room 258A

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Chaired: L. Hedstrom

1:15                        Chair’s Introduction.

1:20      496.1       Sirtuins and Novel Protein Posttranslational Modifications H. Lin. Cornell Univ.

1:45                        Elucidating Anthrax Lethal Factor Interactions with MAP Kinase Kinase Substrates A.B. Goldberg, R. Ngongoni and B.E. Turk. Yale Univ. Sch. of Med. (894.4)

2:00                        Ribozymes to Proteins: Evolution of Enzymes Catalyzing tRNA Processing C.A. Fierke, M.J. Howard, X. Liu, Y. Chen and B.P. Klemm. Univ. of Michigan.

2:25                        Exploiting Non-canonical Amino Acids to Alter Enzyme Substrate Specificity C.L. Windle, A.S. Nelson, A.R. Pearson and A. Berry. Univ. of Leeds and Univ. of Hamburg. (720.1)

2:40                        Active Role of the Substrate during Catalysis by the Therapeutic Enzyme L-Asparaginase II S.B. Rempe, J. Vanegas, A. Anishkin, D.M. Rogers and S. Sukharev. Sandia Natl. Labs., Albuquerque, Univ. of Maryland College Park and Univ. of South Florida. (573.51)

2:55      496.2       Substrate Recognition in Lantibiotic Biosynthesis W. van der Donk. HHMI and Univ. of Illinois at Urbana-Champaign.

3:20                        Discussion.